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Abstract The Nucleocapsid protein (N) of SARS-CoV-2 plays a critical role in the viral lifecycle by regulating RNA replication and by packaging the viral genome. N and RNA phase separate to form condensates that may be important for these functions. Both functions occur at membrane surfaces, but how N toggles between these two membrane-associated functional states is unclear. Here, we reveal that phosphorylation switches how N condensates interact with membranes, in part by modulating condensate material properties. Our studies also show that phosphorylation alters N’s interaction with viral membrane proteins. We gain mechanistic insight through structural analysis and molecular simulations, which suggest phosphorylation induces a conformational change in N that softens condensate material properties. Together, our findings identify membrane association as a key feature of N condensates and provide mechanistic insights into the regulatory role of phosphorylation. Understanding this mechanism suggests potential therapeutic targets for COVID infection. 

Sub-terahertz (THz) wireless communication links require low-SWaP (size, weight, and power) software defined radio (SDR) modems to achieve efficient and reliable data transmission. This research presents the design, development, and experimentation of an SDR system operating in the 135-150 GHz frequency range, utilizing simple I/Q modulation techniques such as differential binary phase shift keying (DBPSK). The system integrates advanced components, including Virginia Diodes (VDI) 110-170 GHz compact upconverter (CCU) and compact downconverter (CCD), high-gain lens horn antennas from Anteral (40 dBi), and the Xilinx RF-SoC ZCU-111 for real time DSP. A 500 MHz IF is implemented on RF-SoC with baseband bandwidth 64 MHz and data rate 64 Mbps via DBPSK modulation. For 20 dBm transmit power at 147 GHz, the nearfield SNR was measured to be 55 dB at 1m lens-to-lens separation for a baseband of 64 MHz. Simulation models of propagation predict 64 Mbps is possibly viable at up to 2 km in a point-to-point connection for a BER of < 10−3. The SDR was realized on the Xilinx PYNQ platform, offering a user-friendly interface while being adaptable to high data rate applications. This digital design is particularly suited for deployment in scenarios such as vehicle-to-vehicle communication, backhaul networks, and data center level interconnects. The research explored challenges related to synchronization, signal integrity, and environmental sensitivity, which are critical for maintaining reliable communication in a 147 GHz channel. A real-time text messaging application demonstrated correct operation of the PYNQ modem.